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<div id="all" class="style1">keywords: <a href="http://www.mbtshoesclearance-online.com/"><strong>MBT Shoes Clearance</strong></a>, MBT Shoes Clearance----&gt;<a href="http://www.mbtshoesclearance-online.com/"><strong>MBT Shoes</strong></a>,MBT Shoes----&gt;<a href="http://www.mbtshoesclearance-online.com/"><strong>MBT Sale</strong></a>,MBT Sale----&gt;<a href="http://www.mbtshoesclearance-online.com/"><strong>MBT Discount</strong></a>,MBT Discount----&gt;----&gt;----&gt;----&gt;----&gt;----&gt;----&gt;----&gt;----&gt;----&gt;----&gt;----&gt;----&gt;----&gt;----&gt;----&gt;<br/><br/>English words count: 183<br/>trans-porter and chromatographic resins (PS and WGA steps) (Table I, values in parentheses). Taking into <a href="http://www.mbtshoesclearance-online.com/"><strong>MBT Shoes</strong></a>,MBT Shoes account the protein recovery* in each purifica-tion step, it is estimated that the overall inactivation of glycine transporter accounts for an 8-fold loss in specific activity, and results in a purification to the calibration curves. The calibration lines are ctructed by plotting the migration of the marker proteins in the H20 and D20 gradients versus their <a href="http://www.mbtshoesclearance-online.com/"><strong>MBT Shoes Clearance</strong></a>, MBT Shoes Clearance known sedimentation coefficients. The partial <a href="http://www.mbtshoesclearance-online.com/"><strong>MBT Discount</strong></a>,MBT Discount specific volume, rc, and the sedimentation coefficient, $2o.w? are calculated according <a href="http://www.mbtshoesclearance-online.com/"><strong>MBT Sale</strong></a>,MBT Sale to the method of Clarke,18 assuming that the partial specific volume of the protein compo-nent is 0.73 ml/g,19 and that the same amount of detergent is bound to the transporter in H20 and D20.ls The following equation is used to calculate the partial specific volume, rc, of the protein-detergent complexes:where the subscripts H and D refer to sucrose density gradients in H20 and D20,.*h and So are the experimentally determined apparent sedimentation coefficients, r/ is the viscosity of the solvent, and p is the density of the solvent, all calculuated as ravcragc as described by Clarke. </div>